Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Evaluating the antidiabetic and antioxidant properties of 5-benzyl-1,3,4-oxadiazole-2-thiol

Shaista Qamar^1,2, Khalid Hussain¹ , Nadeem I Bukhari¹, Sabahat Z Siddique³, Aziz-ur-Rehman ³, Muhammad A Abbasi³, Sajida Parveen¹, Abida Latif¹, Abida Qamar¹, Ejaz Ali¹, Naureen Shehzadi¹, Muhammad Islam¹, Surriya Naheed¹

¹Punjab University College of Pharmacy, University of the Punjab, Allama Iqbal Campus; ²Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences; ³Department of Chemistry, Government College University, Lahore-54000, Pakistan.

For correspondence:- Khalid Hussain Email: khussain.pharmacy@pu.edu.pk Tel:+924299211617

Accepted: 21 April 2019 Published: 31 May 2019

Citation: Qamar S, Hussain K, Bukhari NI, Siddique SZ, A, Abbasi MA, et al. Evaluating the antidiabetic and antioxidant properties of 5-benzyl-1,3,4-oxadiazole-2-thiol. Trop J Pharm Res 2019; 18(5):1095-1100 doi: 10.4314/tjpr.v18i5.26

© 2019 The authors.
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Abstract

Purpose: To evaluate 5-Benzyl-1,3,4-oxadiazole-2-thiol (OXPA) for antidiabetic and antioxidant properties.

Methods: Antidiabetic activity was evaluated using three in vitro models, glucose uptake by yeast cells, alpha amylase inhibition assay and hemoglobin glycosylation inhibition assays. Antioxidant potential was determined by DPPH radical scavenging, reducing power and lipid peroxidation assays.

Results: OXPA showed antidiabetic activity in all the three models. The activity of the compound was comparable with that of metronidazole in glucose uptake by yeast cells, but the alpha amylase inhibition activity of the compound was slightly lower than that of acarbose, whereas the hemoglobin glycosylation inhibition activity of the compound was higher than that of vitamin E. DPPH free radical and hydrogen peroxide scavenging activity of the compound was comparable with that of vitamin C. In reducing power assay, the activity of the compound was lower than that of vitamin C (p > 0.05).

Conclusion: The results of antidiabetic and antioxidant activity indicate that OXPA may be a drug-candidate for treating both diabetes and its associated oxidative stress.

Keywords: Antidiabetic, Glucose uptake, Antioxidant, Reactive oxygen species, Hemoglobin glycosylation, Alpha amylase